Comparison of explant culture methods for human corneal epithelial cell with free-serum
10.13389/j.cnki.rao.2017.0285
- VernacularTitle:无血清条件下组织块法培养人角膜上皮细胞的比较
- Author:
Ling Xiao SHI
1
;
Qian WANG
;
Yan Xiao LU
Author Information
1. 厦门大学附属厦门眼科中心
- Keywords:
corneal epithelial cell;
explant culture;
serum-free;
cell culture
- From:
Recent Advances in Ophthalmology
2017;37(12):1132-1134
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare and analyze the time of cell eruption formation and the rate of cell eruption of human corneal epithelial cells cultured by different explant culture methods under serum-free conditions.Methods Explant tissues were randomly divided into A,B,C and D groups.In A group,the cornea epithelial cells were put side up after stripping corneal endothelium and scraping the corneal epithelium;in B group,the corneal epithelium was put side down after stripping corneal endothelium and scraping the corneal epithelium;in C group,the corneal epithelium was put side up after only stripping corneal endothelium;and in D group,the corneal epithelium was put side down after only stripping corneal endothelium.The morphological changes of the cells in the 4 groups were observed by phase-contrast microscope,and both aforementioned variable were recorded.Results The average time of cell eruption formation and the rate of cell eruption in A,B,C and D group was (7.00 ± 3.00) d and 47.50％ (19/40),(7.55 ±2.58)d and 45.83％ (11/24),(8.43 ±3.32)d and 63.64％ (14/22),(7.49 ± 2.20) d and 72.22％ (39/54),respectively.The chi-square test showed that there was significant difference in the cell eruption rate between A and D group as well as B and D group (all P ＜ 0.05),but there was no significant difference between the other groups (all P ＞ O.05).Conclusion The time of cell eruption formation in the four different explant culture methods was about one week in free-serum conditions.And D group has higher cell eruption rate,and pure plus stable cultured cells when compared with the other three groups.
