Effects of Shexiang Wulong Pills on the expression levels of miR-146a, miR-130 and miR-223 of peripheral blood in patients with RA

VernacularTitle:麝香乌龙丸对RA患者外周血miR-146a、miR-130及miR-223表达水平的影响
Author: wen Zhi WANG ¹ ; song Song WANG ; mei Jian CUI ; Ying CAO ; jie Ding XU ; Qiang YUAN ; han Xiao YU ; hui Chen REN ; jing Wei ZHONG
Author Information

1. 华北理工大学
Keywords: Shexiang Wulong Pills; rheumatoid arthritis (RA); miR-146a; miR-130; miR-223
From: Chinese Traditional Patent Medicine 2017;39(11):2255-2258
CountryChina
Language:Chinese
Abstract: AIM To explore the effects of Shexiang Wulong Pills (Moschus Artifactus,Aconiti Radix Cocta,Pheretima,etc.) drug serum on the expression levels of miR-146a,miR-130 and miR-223 in peripheral blood mononuclear cells (PBMCs) of patients with rheumatoid arthritis (RA).METHODS PBMCs were extracted from blood of 30 cases of RA patients,cells were divided into three groups,blank control group,serum-free groupand Shexiang Wulong Pills drug serum group.After 48 hours,total RNA was extracted,then the expression levels of three miRs were detected by RT-PCR.RESULTS Compared with the serum-free group,the expression levels of miR-146a,miR-130 and miR-223 in the Shexiang Wulong Pills drug serum group were significantly decreased (P ＜ 0.01).CONCLUSION Shexiang Wulong Pills can inhibit inflammation in RA patients by down-regulating the expression levels of miR-223,miR-130 and miR-146a in PBMCs.