Genomic Imbalances in Ependymoma by Degenerate Oligonucleotide Primed PCR-Comparative Genomic Hybridization.
- Author:
Sung Hye PARK
1
;
Gi Jin KIM
;
Min Kyung KIM
;
Hanseong KIM
;
Yoen Lim SUH
;
Sun Hwa PARK
Author Information
1. Department of Pathology, Seoul National University, College of Medicine, Seoul, Korea. parksh@korea.ac.kr
- Publication Type:Original Article
- Keywords:
Ependymoma;
Comparative Genomic Hybridization;
Chromosomal aberration;
PCR
- MeSH:
Chromosome Aberrations;
Comparative Genomic Hybridization;
DNA;
Ependymoma*;
Nucleic Acid Hybridization*;
Polymerase Chain Reaction
- From:Korean Journal of Pathology
2004;38(3):133-137
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: The most consistent chromosomal abnormality in ependymomas, is loss of 22q (17-75%) and gain of 1q (0-50%). However, significance of this abnormality is uncertain. METHODS: Genomic imbalances in 27 Korean ependymomas, including 21 low grade ependymomas, 4 anaplastic and 2 myxopapillary ependymomas, were analyzed by degenerate oligonucleotide primed-PCR-comparative genomic hybridization. RESULTS: Common gains were found in 17 (63%), 20q (59%), 9q34 (41%), 15q24-qter (33%), 11q13 (30%), 12q23 (26%), 7q23-qter (26%), 16q23-qter (30%), 19 (26%), and 1q32-qter (22%). DNA amplification was identified in 12 tumors (44%). Chromosomal loss was a less common occurrence in our study, but was found in 13q (26%), 6q (19%), and 3 (11%). CONCLUSION: The recurrent gains or losses of the chromosomal regions which were identified in this study provide candidate regions that may be involved in the development and progression of ependymomas.
