Protective effects of Huoxue Kangnao Granules on cerebral ischemia-reperfusion injury in rats
10.3969/j.issn.1001-1528.2017.11.003
- VernacularTitle:活血康脑颗粒对大鼠脑缺血再灌注损伤的保护作用
- Author:
yang Jia WAN
1
;
tong Hai WAN
;
Yu HE
;
Li YU
;
hong Jie YANG
;
fen Hui ZHOU
Author Information
1. 浙江中医药大学,浙江杭州310053
- Keywords:
Huoxue Kangnao Granules;
cerebral ischemia-reperfusion injury;
anti-free radical;
anti-inflammatory;
anticoagulation;
antithrombus;
hemorheology
- From:
Chinese Traditional Patent Medicine
2017;39(11):2236-2242
- CountryChina
- Language:Chinese
-
Abstract:
AIM To explore the protective effects of Huoxue Kangnao Granules (Astragali Radix,Morindae officinalis Radix,Puerariae lobatae Radix,etc.) on cerebral ischemia-reperfusion injury in rats.METHODS The rat model for middle cerebral artery occlusion (MCAO) was established,SD rats were randomly divided into sham operation group,model group,positive control group,Huoxue Kangnao Granules low-,middle-and highdose groups.Nerve function score was tested.Volume of cerebral infarction was calculated by TTC staining.SOD,MDA,6-keto-PGF1α,TXB2,IL-1 β and TNF-α were detected by ELISA.The expression levels of MMP-9 and ICAM-1 were detected by RT-PCR.To observe the influence of Huoxue Kangnao Granules on platelet aggregative function,clotting time was analyzed by automatic blood coagulation analyzer,plasma T-PA and PAI activities were determined by ELISA,and hemorheology indices were detected by automatic biochemical analyzer.RESULTS Huoxue Kangnao Granules could significantly decrease cerebral infarction volume,improve nerve function,reduce MDA content,increase SOD activity;increase 6-keto-PGF1 α level,reduce TXB2 content;decrease TNF-α and IL-1β expressions;inhibit MMP-9 and ICAM-1 expressions;extend KPTT and PT;elevate t-PA activity,inhibit PAI activity;decrease whole blood viscosity,enhance erythrocyte deformation ability.Each dosage group of Huoxue Kangnao Granules had a positive correlation between dose and response.CONCLUSION Huoxue Kangnao Granules can protect cerebral ischemia-reperfusion injury in rats,whose mechanisms may be related to anti-free radical injury,anti-thrombus,anti-cerebral vasospasm,improving cerebrovascular permeability,anti-inflammatory,anti-coagulation and improving abnormal changes of hemorheology.
