Simultaneous determination of seven constituents in Chuanbei Luohan Zhike Granules by HPLC-ELSD
10.3969/j.issn.1001-1528.2017.11.017
- VernacularTitle:HPLC-ELSD法同时测定川贝罗汉止咳颗粒中7种成分
- Author:
Min LI
1
;
jun Jian NIE
;
hui Jian WEI
;
xin Zhi ZHENG
Author Information
1. 郑州大学附属医院/南阳市中心医院药学部
- Keywords:
Chuanbei Luohan Zhike Granules;
chemical constituents;
HPLC-ELSD
- From:
Chinese Traditional Patent Medicine
2017;39(11):2304-2307
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish an HPLC-ELSD method for the simultaneous content determination of euscaphic acid,tormentic acid,crategolic acid,corosolic acid,platycodin E,platycodin D3 and platycodin D in Chuanbei Luohan Zhike Granules (Fritillariae cirrhosae Bulbus,Eriobotryae Folium,Platycodonis Radix,etc.).METHODS The analysis of 60％ methanol extract of this drug was performed on a 30 ℃ thermostatic Ultimate XB C18 column (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of acetonitrile-0.5％ ammonium acetate flowing at 0.6 mL/min in a gradient elution manner.RESULTS Seven constituents showed good linear relationships within their own ranges (r ＞ 0.999 0),whose average recoveries were 96.99％-99.78％ with the RSDs of 0.72％-1.72％.CONCLUSION This accurate,simple,specific and reproducible method can be used for the quality control of Chuanbei Luohan Zhike Granules.
