Simultaneous determination of six constituents in Jiaotai Pills by HPLC
10.3969/j.issn.1001-1528.2017.11.018
- VernacularTitle:HPLC法同时测定交泰丸中6种成分
- Author:
jing Li ZHU
1
;
Yang ZONG
;
fen Shu HE
;
Qian ZHANG
;
zheng Wen JU
Author Information
1. 南京中医药大学附属医院,江苏南京210029
- Keywords:
Jiaotai Pills;
epiberberine;
jatrorrhizine hydrochloride;
coptisine hydrochloride;
palmatine chloride;
berberine hydrochloride;
cinnamaldehyde;
HPLC
- From:
Chinese Traditional Patent Medicine
2017;39(11):2308-2311
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish an HPLC method for the simultaneous content determination of six constituents in Jiaotai Pills (Coptidis Rhizoma and Cinnamomi Cortex).METHODS The analysis of 30％ methanol of this drug was performed on a 30 ℃ Agilent ZORBAX SB-C1s column (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of acetonitrile-KH2PO4flowing at 0.8 mL/min in a gradient elution manner,and the detection wavelength was set at 276 nm.RESULTS Epiberberine,jatrorrhizine hydrochloride,coptisine hydrochloride,palmatine chloride,berberine hydrochloride and cinnamaldehyde showed good linear relationships within the ranges of 0.64-41.24 μg/mL (R2 =0.999 9),0.65-43.76 μg/mL (R2 =1.000 0),0.82-52.65 μg/mL (R2 =0.999 9),0.79-50.70 μg/mL (R2 =0.999 9),3.08-197.20 μg/mL (R2=0.999 8) and 0.65-41.65 μg/mL (R2 =0.999 9),whose average recoveries were 98.06％,102.76％,99.27％,99.75％,96.74％ and 101.33％ with the RSDs of 0.56％,0.54％,0.39％,0.55％,0.48％ and 2.14％,respectively.CONCLUSION This accurate,sensitive,stable and reproducible method can be used for the quality control of Jiaotai Pills.
