Simultaneous determination of four constituents in Hydnocarpus anthelmintica by HPLC

VernacularTitle:HPLC法同时测定大风子中4种成分
Author: Xue MA ¹ ; Huan ZOU ; Xu SUN ; ting Yue LI ; gao Shang LIAO ; hong Jun LIU ; jun Yong LI
Author Information

1. 贵州医科大学
Keywords: Hydnocarpus anthelmintica Pierre; luteolin; hydnocarpusol; chrysoeriol; hydnocarpin; HPLC
From: Chinese Traditional Patent Medicine 2017;39(11):2325-2328
CountryChina
Language:Chinese
Abstract: AIM To establish an HPLC method for the simultaneous content determination of four constituents in Hydnocarpus anthelmintica Pierre.METHODS The analysis of 60％ ethanol extract from H.anthelmintica was carried out on a 35 ℃ thermostatic Diamonsil C18column (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of 80％ acetonitrile-0.1％ phosphoric acid flowing at 1.0 mL/min in a gradient manner,and the detection wavelength was set at 347 nm.RESULTS Luteolin,hydnocarpusol,chrysoeriol and hydnocarpin showed good linear relationships within the ranges of 0.0523 6-1.047 μg (r =0.999 9),0.011 24-0.224 8 μg (r =0.999 9),0.029 46-0.589 2 μg (r =0.999 9) and 0.130 5-2.609 μg (r =0.999 9),whose average recoveries were 102.45％ (RSD=1.9％),98.66％ (RSD=1.8％),97.60％ (RSD=1.6％) and 97.88％ (RSD=1.4％),respectively.CONCLUSION This simple,accurate and sensitive method can be used for the quality control of H.anthelmintica.