Effects of an Aerobic Exercise of Different Durations on Nrf2/Keap1 Binding Capacity in Skeletal Muscles of Mice
10.3969/j.issn.1000-6710.2017.10.006
- VernacularTitle:不同时间一次有氧运动对小鼠骨骼肌Nrf2与Keap1结合作用的影响
- Author:
Weixiu JI
1
;
Yayun MAO
;
Linjia WANG
;
Lin LUO
;
Ying ZHANG
Author Information
1. 北京体育大学 北京100084
- Keywords:
acute aerobic exercise;
Nrf2;
Keap1;
skeletal muscle
- From:Chinese Journal of Sports Medicine
2017;36(10):870-874
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of an aerobic exercise of different durations on the binding capacity of NF-E2-related factor 2(Nrf2)/Kelch-like ECH-associated protein-1(Keap1) in skeletal muscle of mice.Methods Thirty C57BL/6J mice were divided into a control(0h) group,an acute exercise for 3 hours (3h) group and an acute exercise for 6 hours(6h) group.The mice ran on treadmill at the speed of 15 m/min for different durations.The mice were sacrificed immediately after exercise and collected skeletal muscles of legs.The high quality fluorescence assay was done to detect the reactive oxygen species(ROS) level in the skeletal muscles of mice.The binding capacity of Nrf2/Keap1 was detected using co-immunoprecipitation.The expression of Nrf2 and Keap1 was analyzed using the Western blotting.Result Compared with group 0h,the Nrf2/Keap1 binding capacity in skeletal muscles of group 3h and 6h decreased significantly(P＜0.05),but the total Nrf2 in skeletal muscles increased significantly(P＜0.05).No significant differences were observed in the expression of total Keapl.The expression of Nrf2 protein of group 3h and 6h increased significantly compared with 0h,with that of group 6h significantly higher than group 3h(P＜0.05).The ROS level in skeletal muscles of group 6h increased significantly compared with group 0h(P＜0.05).Conclusion The effect of acute aerobic exercise on Nrf2/Keap1 binding capacity in skeletal muscle of mice depends on its duration.
