The role of high-mobility group box 1 in magnetic stimulation-induced migration of astrocytes
10.3760/cma.j.issn.0254-1424.2017.10.001
- VernacularTitle:磁刺激诱导下高迁移率族蛋白B1对星形胶质细胞迁移能力的影响
- Author:
Zhe LI
1
;
Jiahong FAN
;
Ganghua GUO
;
Chenxia GUAN
;
Lin YUE
;
Jun GUO
Author Information
1. 郑州大学第五附属医院康复医学科
- Keywords:
Magnetic stimulation;
Astrocytes;
Box 1;
Migration
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2017;39(10):721-726
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the molecular mechanism involved in astrocyte migration induced by magnetic stimulation (MS),and the role of high mobility group box 1 (HMGB1) in migration.Methods Astrocytes were isolated from the cortical tissues of 2-3 day old Sprague-Dawley rats and divided into a stimulus group given MS and a control group without MS.The stimulus group was further divided into an experimental group and a control group,with the former pre-stimulated with 10 μmol/ml U0126 for 30 minutes and no pre-stimulation for the latter.The cells in the experimental group were also randomly divided into siRNA and HMGB1-siRNA transfection groups to examine the role of HMGB1 in astrocyte migration induced by MS.The SiRNA group was transfected with HMGB1 siRNA.Western blotting was used to detect any effect of MS on HMGB1 and extracellular signal-regulated kinase1/2 (ERK1/2).The migration of astrocytes was detected using the scratch assay.Results MS (10 Hz) can promote the phosphorylation of ERK,increase the migration of astrocytes and the expression of HMGB1.After the U0126 treatment and transfection with HMGB1 siRNA,the effects of MS on expression of HMGB1 and migration of astrocytes decreased significantly.Conclusions Magnetic stimulation-mediated migration of astrocytes via activation of the ERK pathway phosphorylation and autocrine of HMGB1.
