Immunoglobulin-like transcript-4 (ILT4) expression in mononuclear cells on the prognosis of sepsis
10.3760/cma.j.issn.1671-0282.2017.10.013
- VernacularTitle:ILT4促进脓毒症炎性反应并介导免疫麻痹
- Author:
Dewen ZHANG
1
;
Xian DING
;
Shuxiong LIU
;
Xinyu XIA
;
Shouchao ZHU
;
Jian HE
Author Information
1. 东方肝胆外科医院急诊科(第二军医大学第三附属医院)
- Keywords:
Sepsis;
Monoyte;
Immunoglobulin-like transcript-4 (ILT4);
CD85;
Major histocompatibility complex class Ⅱ molecules (MHC-Ⅱ);
Interleukin (IL)-6;
Tumor necrosis factor-alpha (TNF-α);
Immunologic paralysis;
Cecal ligation puncture
- From:
Chinese Journal of Emergency Medicine
2017;26(10):1160-1163
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the biological behaviors and effects of immunoglobulin-like transcript-4 (ILT4) expression in mononuclear cells on the prognosis of sepsis.Methods ILT4 +/+ (WT) and ILT4-knockout mice (ILT4-/-) male BALB/c mice were used for sepsis modeling using cecal ligation puncture (CLP).Flow cytometry was used to measure the levels of expression of ILT4 and major histocompatihility complex class Ⅱ molecules (MHC-Ⅱ) in mononuclear cells of peripheral blood 24 h after CLP.ELISA was used to measure the concentrations of interleukin-6 (IL-6) and serum tumor necrosis factor-alpha (TNF-α) in different groups of mice at 0 h,6 h,12 h,and 24 h after CLP to monitor the survival and prognosis over the course of 168 h.Results ILT4 was highly expressed in mononuclear cells of the peripheral blood of septic mice 24 h after CLP in comparison with that before CLP (1292.00 ± 143.70) vs.(193.50 ± 52.54),P ＜ 0.05.MHC-Ⅱ expression in mononuclear cells of the peripheral blood in ILT4-/-mice was significantly higher than that in WT mice (49.38 ± 5.66)％ vs.(24.25 ± 6.76) ％,P ＜ 0.05).Serum IL-6 was significantly elevated 24 h after CLP compared with that before CLP (470.75 ± 88.03) vs.(54.25 ± 20.04),P ＜ 0.05.The serum IL-6 concentration was much lower in ILT4-/-mice thanthatin MT mice (241.25 ± 45.10)vs.(470.75 ± 88.03),P ＜ 0.05;whereas,there was no significant difference in TNF-α expression between two groups of mice (50.88 ± 6.38) vs.(53.13 ± 5.49),P ＞ 0.05.The survival rate of ILT4-/-mice was significantly higher after CLP compared with WT mice (P ＜ 0.05).Conclusion The high level of ILT4 expression in mononuclear cells were observed in peripheral blood during sepsis and it was found to be associated with high serum IL-6 levels and low MHC-Ⅱ expression in mononuclear cells,leading to increased mortality.
