Identification of rodent animals by DNA barcoding technology in Gansu Province
10.3760/cma.j.issn.2095-4255.2017.11.005
- VernacularTitle:DNA条形码技术在甘肃省啮齿动物鉴定中的应用
- Author:
Limin GUO
1
;
Jinxiao XI
;
Yongzhi GAI
;
Kejun MIAO
;
Taoxia MU
Author Information
1. 甘肃省疾病预防控制中心鼠防科
- Keywords:
Rodentia;
DNA barcoding;
Mitochondrial cytochrome C oxidase subunit Ⅰ;
Species identification
- From:
Chinese Journal of Endemiology
2017;36(11):802-805
- CountryChina
- Language:Chinese
-
Abstract:
Objective To apply the DNA barcoding technology for identification of rodent animals and to establish a rodent animal DNA barcode database in Gansu Province.Methods A total of 54 rodent animals were detected.DNA barcoding technology was used to analyze the DNA mitochondrial cytochrome C oxidase subunit Ⅰ (CO Ⅰ) gene sequence in Gansu Province.Results The intra-specific genetic distance was 0-2％ while the interspecific distance ranged from 18％ to 30％.Eight major clusters were apparently showed on a Neighbor joining tree.Conclusion DNA barcoding technology could overcome the shortcomings of the morphological identification,so it could be used to identify the rodent animals and has important implications for disease control and prevention in the natural focus of Gansu Province.
