Study on HPLC Fingerprint of Zhenqi Fuzheng Granules
10.6039/j.issn.1001-0408.2017.33.23
- VernacularTitle:贞芪扶正颗粒的HPLC指纹图谱研究
- Author:
Xiaoyu WEI
1
;
Guodong JIANG
;
Hong CHEN
;
Meihui WANG
;
Jie XU
;
Yu BIAN
;
Wenyue JIANG
Author Information
1. 吉林省现代中药工程研究中心有限公司
- Keywords:
Zhenqi fuzheng granules;
HPLC-ELSD;
Fingerprint;
Quality standard
- From:
China Pharmacy
2017;28(33):4691-4694
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish HPLC fingerprint of Zhenqi fuzheng granules. METHODS:HPLC-ELSD method was ad-opted. The determination was performed on Agilent Zorbax Eclipse XDB-C18 column with mobile phase consisted of acetonitrile-wa-ter(gradient elution)at the flow rate of 1.0 mL/min with column temperature of 40 ℃. The detector evaporation temperature was 50℃,and sample size was 10μL. Using specnuezhenide as reference,HPLC chromatograms of 7 batches of Zhenqi fuzheng gran-ules were determined. The identification and similarity evaluation of common peaks were conducted by using the TCM Chromato-graphic Fingerprint Similarity Evaluation System(2004 A edition). RESULTS:There were 13 common peaks in HPLC chromato-grams of 7 batches of samples. After validated,the similarity of 3 batches of samples in HPLC chromatograms of 7 batches of sam-ples were higher than 0.9,which were in good agreement with control fingerprints. The similarity of 4 batches of samples were <0.9,which were poorly same to control fingerprint. CONCLUSIONS:Established fingerprint can provide reference for quality evalu-ation of Zhenqi fuzheng granules.
