Study on Quality Standard of Miao Medicine Gaultheria yunnanensis
10.6039/j.issn.1001-0408.2017.33.28
- VernacularTitle:苗药透骨香的质量标准研究
- Author:
Chengfen YAO
1
;
Bao ZHANG
;
Dandan XIONG
;
Ting LIU
;
Lin ZHENG
;
Yueting LI
;
Qingfeng CHEN
;
Yongjun LI
Author Information
1. 民族药与中药开发应用教育部工程研究中心/国家苗药工程技术研究中心
- Keywords:
Gaultheria yunnanensis;
TLC;
HPLC;
Quality standard;
Gaultheroside A;
Methyl salicylate
- From:
China Pharmacy
2017;28(33):4711-4714
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the quality standard for Gaultheria yunnanensis. METHODS:TLC was adopted for quali-tative identification of samples. Moisture,total ash and acid-insoluble ash were determined. HPLC method was used to determine the content of methyl salicylate. The determination was performed on Eclipse XDB-C18 column with mobile phase consisted of meth-anol-water(62:38,V/V)at the flow rate of 1.0 mL/min. The detection wavelength was set at 307 nm,and column temperature was 30 ℃. The sample size was 10 μL. RESULTS:TLC spots of samples were clear and well separated. The moisture was 8.2%-10.8%,total ash was 0.9%-4.0% and acid-insoluble ash was 0.1%-0.9%. The linear range of methyl salicylate were 0.045-0.73μg(r=0.9999). RSDs of precision,stability and reproducibility tests were no more than 1.0%. The recoveries of meth-yl salicylate were 97.8%-104.3%(RSD=2.6%,n=9). CONCLUSIONS:The established standard can be used for quality control of G. yunnanensis.
