Simultaneous Determination of 5 Coptis Alkaloid in Lianshen Tonglin Tablets by UPLC
10.6039/j.issn.1001-0408.2017.33.32
- VernacularTitle:UPLC法同时测定连参通淋片中5种黄连生物碱类成分的含量
- Author:
Yanan ZHOU
1
;
Jie BAI
;
Hao YUAN
;
Yongli LIU
Author Information
1. 河北省药品检验研究院
- Keywords:
UPLC;
Coptis alkaloid;
Lianshen tonglin tablets;
Content
- From:
China Pharmacy
2017;28(33):4725-4727
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the method for simultaneous determination of epiberberine,jateorhizine hydrochloride, coptisine,palmatine hydrochloride and berberine hydrochloride in Lianshen tonglin tablets. METHODS:HPLC method was adopt-ed. The determination was performed on Thermo Scientific Syncronis C18 column with mobile phase consisted of acetonitrile-0.1%phosphoric acid solution(pH adjusted to 3.0 with triethylamine)(30:70,V/V)at the flow rate of 0.3 mL/min. The detection wave-length was set at 345 nm,and column temperature was 25 ℃. The sample size was 2 μL. RESULTS:The linear ranges were 4.08-163 ng for epiberberine(r=0.9999),5.72-229 ng for jateorhizine hydrochloride(r=0.9998),5.67-227 ng for coptisine(r=0.9999),6.78-271 ng for palmatine hydrochloride(r=0.9996),13.2-526 ng for berberine hydrochloride(r=0.9999). RSDs of pre-cision,stability and reproducibility tests were lower than 2.0%. The recoveries were 96.83%-103.92%(RSD=2.2%,n=9), 97.62%-104.85%(RSD=2.9%,n=9),99.44%-104.18%(RSD=1.8%,n=9), 98.64%-104.42%(RSD=1.8%,n=9), 98.24%-102.40%(RSD=1.5%,n=9),respectively. CONCLUSIONS:The developed method is simple,sensitive,rapid and accu-rate. It is suitable for simultaneous determination of 5 coptis alkaloids in Lianshen tonglin tablets.
