Inhibitory effect and mechanism of paeoniflorin on proliferation of hypertrophic scar fibroblasts
10.3969.j.issn.1671-7856.2017.11.008
- VernacularTitle:抑制作用及机制研究芍药苷对增殖性瘢痕成纤维细胞增殖
- Author:
Lei WANG
1
;
yuan Yuan MA
;
ling Ya LI
;
min Yue ZHOU
Author Information
1. 河南大学附属淮河医院整形外科
- Keywords:
Paeoniflorin;
Hypertrophic scar;
HS;
Fibroblasts;
Proliferation;
Collagen synthesis
- From:
Chinese Journal of Comparative Medicine
2017;27(11):38-43
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the inhibitory effect and related mechanism of paeoniflorin on proliferation of hypertrophic scar(HS)fibroblasts. Methods HS fibroblasts were cultured with 0(control),200,400,800 μmol/L of paeoniflorin for 24,48,and 72 h. Cell viability was detected by MTT assay. Cell apoptosis was detected using Hoechst staining. Cell cycle was measured by flow cytometry. The levels of type I collagen(COL I)and type III collagen(COL III)were detected by enzyme linked immunosorbent assay(ELISA). The expressions of transforming growth factor-β (TGF-β)/Smad signaling pathway related proteins, as well as matrix metalloproteinase 1(MMP1)and MMP13 were detected by Western blot. Results 200, 400, 800 μmol/L of paeoniflorin reduced the cell viability of HS fibroblasts significantly(P < 0.01), with the nuclei turning pale and shrunken, and caused cell cycle arrest at G1 phase(P <0.01). Moreover, the levels of COL I and COL III in the cells were decreased significantly(P < 0.01), and the expressions of MMP1, MMP13, TGF-β1, p-Smad2 and p-Smad3 were down-regulated significantly(P < 0.01). Conclusions Paeoniflorin can obviously inhibit the proliferation and collagen synthesis of hypertrophic scar fibroblasts,probably through inhibition of the TGF-β1/Smad signaling pathway.
