IL-37b alleviates septic shock through inhibition of immune responses related to dendritic cells
10.3969.j.issn.1671-7856.2017.11.009
- VernacularTitle:IL-37 b通过抑制树突状细胞相关的免疫应答缓解感染性休克
- Author:
yuan Meng LI
1
;
qiang Zheng YAN
;
fei Rong WEI
;
jiu Xing YANG
;
min Rui ZHU
;
Ran GAO
Author Information
1. 中国医学科学院医学实验动物研究所
- Keywords:
IL-37b;
Purification;
Dendritic cells;
Immune responses;
Septic shock
- From:
Chinese Journal of Comparative Medicine
2017;27(11):44-49
- CountryChina
- Language:Chinese
-
Abstract:
Objective To express the recombinant IL-37b protein and to investigate its role in the regulation of immune responses. Methods The prokaryotic expression vector pET28/IL-37b was constructed. The recombinant IL-37b protein was induced to be expressed and was purified using Ni2+-NTA gel column. C57BL/6 J mice were treated with IL-37b and injected with lipopolysaccharide(LPS)to induce septic shock,and the expression levels of IL-1β,IL-6,IFN-γ in the serum of the mice were detected. Dendritic cells from bone marrow of the mice were isolated and cultured, and were treated with IL-37b. After LPS-induced activation, the expression levels of marker molecules such as CD40, CD80 and MHCII on the cell surface, and cytokines such as IL-1β, IL-10, IL-12 and TNF-α in the culture supernatants were detected by flow cytometry. The CD4 +T cells from mice were isolated and the inhibitory effects of IL-37b on the expression of IFN-γ,TNF-α and IL-10 in the culture supernatants of T cells after induction by LPS were detected. Results IL-37b reduced the expression levels of proinflammatory cytokines in the serum of septic shock mice. IL-37b also inhibited the expression of co-stimulatory molecules and proinflammatory cytokines of the mouse dendritic cells, and suppress the activation of CD4 +T cells in vitro. Conclusions Purified recombinant IL-37b protein has high bioactivity, and can alleviate septic shock in organisms through inhibiting the activation of dendritic cells and related T cell immune responses.
