The expression and effect of LINGO-1 during the differentiation of spinal cord derived neural stem cells
10.3969/j.issn.2095-4344.2017.33.023
- VernacularTitle:LINGO-1在脊髓神经干细胞分化过程中的表达特征及效应
- Author:
Gang XU
1
;
guang Chen ZHAO
;
Wei SUN
;
Fen JU
;
Hua YUAN
;
Xiang MOU
Author Information
1. 解放军新疆军区总医院全军骨科中心
- From:
Chinese Journal of Tissue Engineering Research
2017;21(33):5394-5399
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: It is of great significance to explore the expression and effect of LINGO-1 in the differentiation of spinal cord derived neural stem cells (SpNSCs) for regulating neural stem cell differentiation and repairing spinal cord injury. OBJECTIVE: To investigate the expression features and biological effects of LINGO-1 in the differentiation of SpNSCs. METHODS: SpNSCs were isolated from the rat spinal cord and cultured in vitro. The expression characteristics of LINGO-1 was observed through double immunofluorescence staining of LINGO-1 and Nestin (neural stem cells), β-Tubulin III (neurons), GFAP (astrocytes) and O4 (oligodendrocyte) at 0-5 days of differentiation. SpNSCs isolated from the rat spinal cord were cultured in vitro and divided into siRNA group and control group. The siRNA group was transfected with LINGO-1 shRNA lentiviral vector to down-regulate the expression of LINGO-1, and the control group was transfected with Scramble-shRNA lentiviral vector. The growth of neurites was detected by immunofluorescence staining at 5 days after transfection.RESULTS AND CONCLUSION: The SpNSCs could differentiate into neurons, astrocytes and oligodendrocytes. LINGO-1 was expressed in SpNSCs, neurons and oligodendrocytes, but not in astrocytes. The neurite length of the siRNA group was significantly longer than that of the control group (P < 0.05). In summary, the SpNSCs have the potential of multi-directional differentiation, and LINGO-1 has a negative effect on the neurite growth.
