Simultaneous Determination of 5 Components in Shuganning Injections by HPLC
10.6039/j.issn.1001-0408.2017.33.26
- VernacularTitle:HPLC法同时测定舒肝宁注射液中5种成分的含量
- Author:
Xuran ZHI
1
;
Mi WANG
;
Haojing SONG
;
Zhanjun DONG
Author Information
1. 河北省人民医院药学部
- Keywords:
HPLC;
Shuganning injections;
Chlorogenic acid;
Geniposide;
Baicalin;
Baicalein;
Scutellarin;
Content
- From:
China Pharmacy
2017;28(33):4702-4705
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop a method for the simultaneous determination of 5 components in Shuganning injections. METHODS:HPLC method was adopted. The determination was performed on Symmetry? C18 column with mobile phase consisted of methanol-0.4% phosphoric acid(gradient elution)at the flow rate of 1.0 mL/min. The detection wavlengths were set at 238 nm (geniposide,baicalin) and 327 nm (chlorogenic acid,baicalein,scutellarin). The column temperature was 30 ℃ and the sample size was 10 μL. RESULTS:The linear ranges were 0.4062-26.0 μg/mL for chlorogenic acid(r=0.9999),2.5000-160.0 μg/mL for geniposide (r=0.9999),6.5620-420.0 μg/mL for baicalin (r=0.9999),0.3125-20.0 μg/mL for baicalein (r=0.9996), 0.5859-37.5 μg/mL for scutellarin (r=0.9998). The limits of quantify were no higher than 31.20 ng,limits of detection were no higher than 15.60 ng. RSDs of precision, stability and reproducibility tests were lower than 2.0% ;the recoveries were 97.72%-101.10%(RSD=1.21%,n=6),97.67%-102.40%(RSD=1.87%,n=6),97.64%-101.10%(RSD=1.31%,n=6), 96.45%-100.10%(RSD=1.47%,n=6),96.16%-101.10%(RSD=1.69%,n=6),respectively. CONCLUSIONS:The method is simple,precise,stable and reproducible,and can be used for simultaneous determination of 5 components in Shuganning injection.
