Fluorescent staining with hypersensitivity and enhancement for early diagnosis of superficial fungal infection
10.13602/j.cnki.jcls.2017.10.04
- VernacularTitle:特敏增强荧光染色法在浅部真菌病诊断中的应用
- Author:
peng Yun LUO
1
;
min Yang GAO
;
Yun JIN
;
Qing JIANG
;
Jie LI
;
yi Xin FAN
;
Ping ZHAN
Author Information
1. 江西省皮肤病专科医院真菌科
- Keywords:
hypersensitive,enhanced fluorescent staining;
fungal direct microcopic examination;
potassium hydroxide based smear assay;
calcofluor white staining
- From:
Chinese Journal of Clinical Laboratory Science
2017;35(10):736-738
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the application of novel fluorescent staining method with hypersensitivity and enhancement for microscopic examination of superficial fungal.Methods A total of 200 cases with clinically suspected superficial fungal infection were screened by the hypersensitive,enhanced fluorescent staining,calcofluor white staining and microscopic examination with 1.78 mol/L potassium hydroxide (KOH) based smear assay,respectively.The positive percentages of the different methods were calculated and compared.Results In the developed fluorescent staining examination,clean background was shown in the view field.The hyphae and spores displayed in bright blue,thus fungal morphology was so distinct that they were easy to be distinguished.Compared with calcofluor white staining and KOH based smear,the fluorescent staining showed high positive rate with significant difference respectively (x2 =17.984,P ＜ 0.05;x2 =32.063,P ＜ 0.05).Conclusion The novel fluorescent staining should be a rapid,accurate method for microscopic examination of fungi,which is worthy to be widely used in clinical laboratories for early diagnosis of fungal infection.
