Study on frequency of 9 bp sequence deletion of mithochondrial DNA in three ethnic nationalities of Guizhou Province
10.3969/j.issn.1671-8348.2017.33.031
- VernacularTitle:贵州省3个少数民族线粒体DNA 9 bp序列缺失频率的分析研究
- Author:
Xuehong GUO
1
;
Yan HE
;
Wangde ZHANG
;
Chanjuan WANG
;
Ting ZHANG
;
Zhizhong GUAN
Author Information
1. 地方病与少数民族性疾病教育部重点实验室/贵州医科大学分子生物学重点实验室
- Keywords:
Guizhou;
Buyi nationality;
Shui nationality;
Miao nationality;
mtDNA
- From:
Chongqing Medicine
2017;46(33):4702-4704
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the frequency of 9 bp sequence deletion of mithochondrial DNA in three ethnic nationalities of Guizhou Province .Methods A total of 183 male blood samples were randomly extracted from Miao nationality (69 cases) in Leishan County ,Shui nationality (44 cases) and Buyi nationality (70 cases) in Libo County .Polymerase chain reaction(PCR) and di-rect sequencing were used to detect the mithochondrial DNA 9 bp sequence deletion .Results The standard type ,deleting type and 3 type were found in the samples of 3 ethnic nationalities in Guizhou Province ,the highest deletion frequency was Shui nationality (40 .91% ) in Libo County ,1 cases of 3 type was detected in Miao nationality of Leishan County ,and the mtDNA 9 bp deletion fre-quency had no statistical difference among 3 ethnic nationalities(P>0 .05) .Conclusion The frequency of mtDNA 9 bp deletion is different among three native minorities ,the genetic variation in Shui nationality is larger ,compared with Miao nationality genetic re-lationship ,Shui nationality has a relatively closer affinity with Buyi nationality .
