Correlative analysis of three sperm indexes and IVT-ET outcome after semen treatment
10.3969/j.issn.1671-8348.2017.32.018
- VernacularTitle:精液处理前、后精子3项对IVT-ET结局的相关性分析
- Author:
Shaowei CHEN
1
;
Mingyong WANG
;
Li FU
;
Ying Gui HUANG
;
Fang WANG
;
Shuang LIU
;
Xiguang MAO
Author Information
1. 西南医科大学附属医院生殖医学技术部
- Keywords:
assisted reproductive technology;
sperm parameter;
IVF outcome;
correlation coefficient
- From:
Chongqing Medicine
2017;46(32):4526-4528,4532
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the correlation between three sperm indexes and the outcome of in vitro fertilization and embryo transfer(IVT-ET) before and after semen treatment.Methods The semen derived from 298 male patients treated with IVT-ET from the Affiliated Hospital of Southwest Medical University was retrospectively analyzed.Routine analysis of semen before treatment was performed according to the WHO standard.Vitrolife density gradient centrifugation combined with upstream method was used to analyze sperm parameters after sperm analysis,and the observation and statistics were performed after fertilization and sequential culture to day 3.Results The total viability of spermatozoa before and after semen treatment,the percentage of normal spermatozoa,sperm density,and other differences were statistically significant (P＜0.05);The normal sperm morphology rate was positively correlated with fertilization rate(r=0.487,P＜0.01) and cleavage rate(r=0.250,P＜0.05);The sperm vitality of post-processed semen was positively related to the fertilization rate (r =0.249,P＜0.05,);and significantly cleavage rate (r =0.272,P＜0.05);After treatment,sperm density was positively correlated with 2 PN(r=0.609,P＜0.01) and multiple PN fertilization rates(r=0.243,P＜0.05).Conclusion Our experimental results indicate that the parameters of post-processed sperm treated at the day when the oocytes were collected may be more helpful in predicting the fertilization rate and cleavage rate of IVF-ET.Therefore,the IVF-ET treatment protocols should be chose based on the normal sperm morphology rate and sperm vitality after semen treatment.
