Preparation of myocardial ischemia/reperfusion injury model by overlapping line in mice
10.3969/j.issn.1671-8348.2017.32.024
- VernacularTitle:套线技术制备小鼠心肌缺血/再灌注损伤模型
- Author:
Zhongshen JIA
1
;
Zhi JIANG
;
Anjie LI
;
Yueting WU
;
Fang WEI
Author Information
1. 贵州医科大学附属人民医院心内科
- Keywords:
myocardial infarction;
myocardial ischemia;
myocardial reperfusion injury;
animal model;
mice,inbred C57BL
- From:
Chongqing Medicine
2017;46(32):4546-4549
- CountryChina
- Language:Chinese
-
Abstract:
Objective To improve the operation speed and reliability of the myocardial ischemia/reperfusion injury(I/R) model in mice.Methods The operative time and the survival rate of the three models were compared.The postoperative leukocyte count and serum IL-6 level were detected to evaluate the inflammatory response.The infarct size was assessed by TTC/Evans blue staining.The left ventricular end diastolic pressure and the pressure change rate were measured by a pressure catheter for the evaluation of cardiac function.Intraditional method,open chest surgery was performed to ligate and loose the left coronary artery(LCA)under the assistance of intubation and ventilation.In novel method,isoflurane was used for anesthesis,and then heart was squeezed out of the chest to ligate and loose LCA with a slipknot.In.overlapping line method,the heart was also squeezed out,but a square knot was performed to ligate LCA and a 2-0 nylon together,and then the nylon thread was drew out to loose LCA.Results Compared with traditional method,the novel method and overlapping line method had significant short operation time(P＜0.01 or P＜0.05),less white blood cell count and serum IL-6 concentration(P＜0.05) and higher mice survival rate(P＜0.01);compared with novel method,the overlapping line method further simplified the reperfusion procedure and decreased total procedure time(P＜0.01).There was no significant difference in cardiac infarct size and cardiac function by using the three methods(P＞0.05),but the overlapping line method had less coefficient of variation compared to novel method.Conclusion Compared with the novel method,the overlapping line method reduces the operation difficulty,shortens the operation time and improves the stability of I/R model.
