Experience of Simplified Protocols in In-Vitro Fertilization and Embryo Transfer Program.
- Author:
Hyuck Chan KWON
1
;
Kyung Joo HWANG
;
Hyun Won YANG
;
Jung Hyun RHYU
;
Soon Jung HONG
;
Jee Young PARK
;
In Ja YOON
;
Dong Min KIM
;
Chan Il PARK
;
Haeng Soo KIM
;
Ki Suk OH
Author Information
1. Department of Obstetrics and Gynecology, Ajou University School of Medicine, Suwon, Korea.
- Publication Type:Original Article
- Keywords:
IVF-ET;
GnRH agonist;
simplification;
serum-free culture media;
synthetic serum replacement;
micronized progesterone
- MeSH:
Culture Media, Serum-Free;
Embryo Transfer*;
Embryonic Structures*;
Estradiol;
Female;
Fertilization*;
Humans;
Injections, Intramuscular;
Insemination;
Menstrual Cycle;
Oocyte Retrieval;
Ovulation Induction;
Pregnancy Rate;
Progesterone;
Retrospective Studies;
Ultrasonography
- From:Korean Journal of Obstetrics and Gynecology
1997;40(10):2119-2129
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
During the past few years much effort has been put into simplifying the clinical man-agement of in-vitro fertilization/embryo transfer cycles. One important step was the intro-duction of transvaginal ultrasound-guided oocyte collection, as previously described. This study describes further simplifications in the clinical management of ovarian stimulation and luteal support, and in-vitro fertilization procedure. During the period from October 1994 to September 1995, two major simplification steps were introduced. All cycles were administe-red with a gonadotrophin-releasing hormone agonist according to a long or short protocol preventing premature LH surge. During period I (Group I, n=62 cycles), closer monitoring by several pelvic ultrasound scans and serum oestradiol was used for monitoring the ovarian stimulation ; HTF media with fetal cord serum was used for insemination, growth and tran-sfer media in IVF-ET procedure ; progesterine in oil was daily used by intramuscular injec-tion for luteal support. During period II(Group II-I, n=71 cycles), only several ultrasound scans were used for monitoring the ovarian cycle ; Medi-cult IVF media containing synthetic serum replacement was used for insemination, growth and transfer media; Progesterine in oil was used daily by intramuscular injection for luteal support. During period III(Group II-II, n=16 cycles), further simplification of the clinical management was introduced by using a intravaginal micronized progesterone(Utrogestan) for luteal support. Retrospective analysis between Group I and Group II showed no differences in the number of oocyte(13.2+/-0.8/14.6+/-1.0), fertilization rate(71.5 %/60.7 %), cleavage rate(63.6 %/57.9 %), number of embryos transfered(5.0+/-0.5/4.5+/-0.5). Ongoing pregnancy rates obta ined from the three groups(Group I, Group II-1, Group II-II) were 25.8 %, 25 % and 40 %, respectively(p=ns). But introduction of minimal monitoring gave a significant reduction in the average number of US measurements in the simplified groups(Group II) compared with the group using the conventional monitoring protocol(Group I)(3.8+/-1.0/8.7+/-2.8, p<0.05). In the above groups, five patients developed severe OHSS but there was no differenc e in the distribution. Conclusively, simplified protocols including minimal follicle monitoring only by US, IVF-ET with Medi-cult IVF media containing synthetic serum replacement and the luteal support with intravaginal micronized progesterone gave a increased efficacy of the clinical phase of IVF treatment without a reduction in the success rate.
