Effect of Compound Jiegu Tablets and Zhuanggu Tablets on the differentiation of bone marrow mesenchymal stem cells in vitro
10.3969/j.issn.2095-4344.2017.33.003
- VernacularTitle:骨髓间充质干细胞体外分化过程中复方接骨片和壮骨片含药血清的作用
- Author:
Bo WU
1
;
Ye LIU
;
Xu MA
;
yuan Su LIU
;
han Yi WANG
;
yuan Jia WANG
;
bo Cheng TAN
;
bo Zheng YANG
Author Information
1. 沈阳市骨科医院关节外科
- From:
Chinese Journal of Tissue Engineering Research
2017;21(33):5262-5267
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) are a kind of pluripotent stem cells in the bone marrow. Drug-containing serum treatment may be followed by a variation in the expression of genes involved in multiple signaling pathways. OBJECTIVE: To study the effect of compound Jiegu Tablets and Zhuanggu Tablets on the differentiation of BMSCs in vitro. METHODS: Sixty Sprague-Dawley rats were randomized into Jiegu group, Zhuanggu group, combined group and blank control group. Different drug-containing sera were prepared and used to culture BMSCs isolated from the rat bone marrow. RT-PCR and western blot assay were used to detect the expression of BMP-2, Runx2, VEGF and Akt at mRNA and protein levels, respectively, at 24 hours after culture.RESULTS AND CONCLUSION: Compared with the blank control group, the expression levels of Akt and VEGF mRNA and protein were significantly increased in the Jiegu group and combined group (P < 0.05 or P < 0.01), but showed no difference in the Zhuanggu group (P > 0.05). Compared with the blank control group, the expression levels of BMP-2 and Runx2 mRNA and protein were significantly increased in the Jiegu group (P < 0.05) and very significantly increased in the Zhuanggu group and combined group (P < 0.01). These findings indicate that compound Jiegu Tablets and Zhuanggu Tablets containing sera lead to synergistic effects on the BMSCs differentiation into vascular endothelial cells and osteoblasts in vitro.
