Tryptophan metabolism change in patients with chronic hepatitis B and hepatitis B cirrhosis and its correlation analysis
10.3969/j.issn.1671-8348.2017.33.005
- VernacularTitle:慢性乙型肝炎及乙型肝炎性肝硬化患者色氨酸代谢变化及其相关性分析
- Author:
Yanna BAN
1
;
Qianna ZHEN
;
Linlin DENG
;
Yifan HE
;
Min DING
Author Information
1. 重庆医科大学附属第一医院生殖健康与不孕症专科实验室 400016
- Keywords:
tryptophan;
kynurenine;
5-hydroxy tryptamine;
chronic hepatitis B;
hepatitis B cirrhosis
- From:
Chongqing Medicine
2017;46(33):4622-4624,4627
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the tryptophan (Trp) metabolism change in the patients with chronic hepatitis B (CHB) and hepatitis B cirrhosis (HBC) and to analyze the related risk .Methods Eighty-one cases of CHB and 40 cases of HBC hospitalized in the First Affiliated Hospital of Chongqing Medical University from October 2015 to January 2016 served as the stud-y subjects ,and 40 healthy persons as the controls .High performance liquid chromatography -fluorescence detection (HPLC-FD) were adopted to detect the levels of plasma Trp ,kynurenine (Kyp) and its metabolite 5-hydroxy tryptamine (5-HT) .HBV-DNA vi-ral replication number was detected by real-time quantitative PCR .The serological indicators differences among 3 groups were ana-lyzed .The risk factors were analyzed and screened by the Logistic regression analysis and Pearson correlation analysis .Results Compared with the control group ,plasma 5-HT and Trp levels in the CHB group and HBC group were significantly decreased (P<0 .01);plasma Kyn/Trp in the HBC group was significantly higher than that in the other two groups (P<0 .01) .Kyn/Trp in the HBC group was negatively correlated with A/G (r= -0 .686 ,P<0 .01) ,and Kyn/Trp in the CHB group was positively correlated with Log DNA (r= 0 .784 ,P<0 .01) .The Logistic regression analysis indicated that Kyn/Trp was the risk factor of CHB and HBC .Conclusion Detecting plasma Trp and its metabolite can serve as the adjuvant indicators for assessing the progression of hep-atitis B .
