Hypoxia Increases the Expression of ICAM-1 in Cultured Human Synovial Fibroblasts.
- Author:
Hyun Soon JEON
1
;
Hong Sun BAEK
;
Byung Yun HWANG
;
Wan Hee YOO
Author Information
1. Department of Internal Medicine, Chonbuk National University Medical School and Research Institute of Clinical Medicine, Chonju, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
Hypoxia;
ICAM-1;
Synovial fibroblasts
- MeSH:
Adhesives;
Anoxia*;
Cytokines;
Fibroblasts*;
Humans*;
Intercellular Adhesion Molecule-1*;
Interleukin-1;
Lymphocytes;
RNA, Messenger;
Tumor Necrosis Factor-alpha
- From:The Journal of the Korean Rheumatism Association
2002;9(1):23-33
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: Hypoxic conditions are thought to be exist in inflamed arthritic synovium.Several in vitro and in vivo studies indicate that hypoxia can initiate events that lead to pro-adhesive changes.Therefore,this study was designed to examine the effects of hypoxia on the expression of ICAM-1 by cultured human synovial fibroblasts. METHODS: Synovial fibroblasts were isolated from patients with RA and cultured at hypoxic condition.To quantify the expression of ICAM-1 mRNA in synovial fibroblasts,RT-PCR was performed.The levels of cytokines in culture supernatants were measured by ELISA.The activation of NF-
