The Therapeutic Effect of Quercetin-loaded PLGA-TPGS Nanoparticles on the Hepatocarcinoma Ectopic Solid Tumor-bearing Mice
10.12007/j.issn.0258-4646.2017.09.006
- VernacularTitle:槲皮素PLGA-TPGS纳米粒对小鼠肝癌异位实体瘤治疗效果的研究
- Author:
Hong XU
1
;
Chenghong ZHANG
;
Xin GUAN
;
Hao DONG
;
Rongqian XU
;
Yu CHEN
;
Meng GAO
;
Yan TIAN
Author Information
1. 大连医科大学基础医学院机能学实验室
- Keywords:
Quercetin;
nanoparticles;
mice;
tumor;
HCa-F hepatocarcinoma cells;
tumor growth inhibition rate
- From:
Journal of China Medical University
2017;46(9):791-795
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the therapeutic effects of Quercetin (QT)-loaded PLGA-TPGS nanoparticles (QPTN) on solid tumor-bearing mice with HCa-F hepatocarcinoma in vivo.Methods The model of HCa-F hepatocarcinoma solid tumor-bearing mice was established by implanting HCa-F cells into 48 mice.The mice were divided into 6 groups randomly:the negative control,empty PLGA-TPGS nanoparticles,5-Fluorouracil solutions (FS),Quercetin solutions (QTS),QT-loaded PLGA nanoparticles (QPN),and QPTN groups.Each group was treated using tail vein twice a day for 20 days;then,all mice were sacrificed.The increment tumor volumes and tumor growth inhibition rate were counted.Then,tumor specimens were prepared for hematoxylin & eosin (HE) staining and observed under a microscope.Results The results showed that the increment tumor volumes of mice in the QPTN,QPN,and FS groups were significantly smaller than that in the negative control group (P < 0.05 or P < 0.01).The tumor growth inhibition rate of the QPTN group was 59.07%,which was much higher than that of the QTS group (23.94%),the FS group (35.14%),and the QPN group (46.14%).The results of the HE staining on the tumor sections also indicated that the QPTN group showed a better therapeutic outcome compared to the other groups.Conclusion The QPTN has a better therapeutic effect on the model of solid tumor using HCa-F cells-bearing mice than the QPN,QTS,and FS.