Neuraminidase gene profiling of influenza B virus in Shangrao

VernacularTitle:上饶市乙型流感病毒耐药基因分析
Author: Yanyan ZHANG ¹ ; Yu LAI ; Lin ZHU ; Ying XIONG ; Jianxiong LI ; Changhe CHENG
Author Information

1. 上饶市疾病预防控制中心检验科
Keywords: influenza B virus; resistance; neuraminidase gene
From: Chinese Journal of Infection and Chemotherapy 2017;17(5):538-540
CountryChina
Language:Chinese
Abstract: Objective To characterize the neuraminidase (NA) genes of influenza B virus in Shangrao.Methods The specimens of nasopharyngeal swabs were collected from patients with influenza-like symptoms in influenza sentinel hospital.Seven strains of influenza B virus were randomly selected for culture and isolation in Madin-Darby Canine Kidney Epithelial Cells (MDCK Line).Viral RNA was extracted.Fragments of NA genes were amplified by one-step RT-PCR and then were sequenced.The data obtained were analyzed with software DNAStar 6.0 and Mage 5.0.The deduced amino acid sequences were examined to explore the features ofNA gene.Results The NA gene showed high homology ofnucleotides between the 7 strains of influenza B virus.No amino acid substitution was found in catalytic or framework residues of the deduced amino acid sequences of NA gene.Conclusions All the 7 strains of influenza B virus were sensitive to neuraminidase inhibitors.However,ongoing resistance surveillance is necessary for control and prevention of influenza.