The change of Porphyromonas gingivalis in rat gingival crivicular fluid under chronic intermittent hypoxia
10.3969/j.issn.1001-3733.2017.05.002
- VernacularTitle:慢性间歇性低氧条件下大鼠龈沟液中牙龈卟啉单胞菌的变化
- Author:
Tiantian ZHENG
1
;
Xiaoqin WANG
Author Information
1. 山西医科大学
- Keywords:
Chronic intermittent hypoxia;
Periodontitis;
Porphyromonas gingivalis;
Real-time PCR
- From:
Journal of Practical Stomatology
2017;33(5):580-583
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the change of Porphyromonas gingivalis (P.gingivalis) in rat gingival crivicular tluid (GCF) under chronic intermittent hypoxia.Methods:32 male SD rats were randomly divided into 4 groups (n =8):normoxia control group (A),normoxia with periodontitis group (B),intermittent hypoxia group(C),and intermittent hypoxia with periodontitis (D).Periodontitis model was established by orthodontic silk ligation at the maxillary second molar neck and high sugar diet.The rats in normoxia and hypoxia group were raised respectively under the condition of ordinary oxygen and chronic intermittent hypoxia respectively.After 8 weeks,GCF of the target teeth was collected,P.gingivalis was quantified by real-time fluorescent quantitative PCR.Results:P.gingivalis was detected in all groups.P.gingivalis in group D was more than in other groups(P ＜0.05);meanwhile,P.gingivalis in group B was more than in group A(P ＜0.05).Conclusion:Chronic intermittent hypoxia can aggravate the severity of periodontitis,which is associated with the increase of P.gingivalis in GCF.
