The effects of hyaluronic acid and TGF-β1 on the growth of mandibular condylar cartilage and the hyperthophic differentiation of the condylar chondrocyts
10.3969/j.issn.1001-3733.2017.05.003
- VernacularTitle:透明质酸、TGF-β1对下颌骨髁突软骨增殖分化的影响
- Author:
Dongpeng LIN
1
;
Jinru LV
;
Tianyi ZHAO
;
Yonggong WANG
;
Liwei PENG
;
Qin MA
Author Information
1. 河南省人民医院口腔颌面外科
- Keywords:
Mandibular condylar cartilage;
Tissue culture;
In vitro;
Hyaluronic acid(HA);
TGF-β1;
Hyperplasia
- From:
Journal of Practical Stomatology
2017;33(5):584-588
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of hyaluronic acid(HA) and TGF-β1 on the growth of mandibular condylar cartilage and the hyperthophic differentiation of the condylar chondrocyts.Methods:60 condyle samples from newborn mice were in vitro cultured and treated with HA(0.5 mg/ml),TGF-beta 1 (5 ng/ml) and without additional agent(the control) respectively.The Morphological observation,Alizarin Red Staining,Alkaline phosphatase staining and condylar cartilage surface area measurement were conducted after 1,2,4,6 and 8 weeks of culture respectively.Results:High-density photoresist area was observed in the condylar cartilage of the control group after 4 weeks of culture.Alizarin Red Staining and Alkaline phosphatase staining showed condylar cartilage matrix production and calcification.The HA group showed no high-density photoresist area at all time points,however,the cartilage area was significantly increased (P ＜ 0.05);the TGF-beta 1 group showed high-density photoresist area after 2 weeks of culture.but the cartilage area were not significantly changed(P ＞ 0.05).Conclusion:HA can promote the growth of condylar cartilage in vitro,but have an inhibitory effect on chondrocyte differentiation.TGF-β1 plays a role in mandibular condylar chondrocyte hypertrophic differentiation in the early days of in vitro culture.
