Role of miR-126 in the Biological Behavior of HL-60 Cell Line
10.12007/j.issn.0258-4646.2017.09.007
- VernacularTitle:miR-126对HL-60细胞系增殖和凋亡等生物学行为的影响
- Author:
Heyang ZHANG
1
;
Rui ZHANG
;
Pingping WANG
;
Yue WANG
;
He WANG
;
Yan LI
Author Information
1. 中国医科大学附属第一医院血液科
- Keywords:
miR-126;
leukemia;
proliferation;
apoptosis;
acute promyelocytic leukemia
- From:
Journal of China Medical University
2017;46(9):796-801,806
- CountryChina
- Language:Chinese
-
Abstract:
Objective To cultivate human leukemia cells (HL-60) which were transiently transfected either a miR-126 mimic or inhibitor,and then to characterize the proliferation and apoptotic behavior of the transfected leukemia cells.Methods The leukemia cell line was developed and RT-PCR was performed to evaluate miR-126 expression levels.An instantaneous plasmid tmnsfection technique was used to transfect cells with either the miR-126 mimic or inhibitor.CCK-8,FCM,and clone formation tests were performed to analyze the proliferative and apoptotic behaviors of the leukemia cells.Results Proliferation was significantly decreased in cells transfected with the miR-126 mimic for 0,24,48,and 72 hours (P ＜ 0.05).Specifically,the G1,S,and G2 phases were significantly inhibited (P ＜ 0.05),the late and early apoptosis (UR+LR) rate increased (P ＜ 0.05),and the average rate of colony formation was also significantly decreased (P ＜ 0.05).Additionally,proliferation was significantly increased in cells transfected with the miR-126 inhibitor for 0,24,48,and 72 hours (P ＜ 0.05).Specifically,the G1,S,and G2 phases were increased (P ＜ 0.05),the UR + LR decreased significantly (P ＜ 0.05),and the average rate of colony formation was significantly increased (P ＜0.05).Conclusion In HL-60 cells,miR-126 can inhibit proliferation and promote apoptosis;thus,miR-126 may play an important role in the occurrence and development of leukemia as a tumor-suppressor miRNA.
