The osteogenesis ability of human umbilical cord Wharton's Jelly-derived mesenchymal stem cells and periodontal mesenchymal stem cells

VernacularTitle:人脐带Wharton's Jelly来源间充质干细胞与人牙周膜干细胞成骨分化能力的对比研究
Author: Yinli HUANG ¹ ; Hong ZHOU ; Xiaoxia SU ; Tianyu ZHONG
Author Information

1. 西安交通大学口腔医学院正畸科
Keywords: Human umbilical cord Wharton's Jelly-derived mesenchymal stem cells (hUCWJMSCs); Human periodontal ligament mesenchymal stem cells (hPDLSCs); Osteogenic differentiation
From: Journal of Practical Stomatology 2017;33(5):669-673
CountryChina
Language:Chinese
Abstract: Objective:To compare the osteogenesis ability between human umbilical cord Wharton's Jelly-derived mesenchymal stem cells(hUCWJMSCs) and human periodontal ligament mesenchymal stem cells (hPDLSCs) in vitro.Methods:hUCWJMSCs and hPDLSCs were in vitro cultured.The cell proliferation capacity was examined by MTT assay.After osteogenesis induction culture,ALP activity of the cells was determined,minerialization was observed by alizarin red staining,OPN and Runx2 mRNA expression was analyzed by Real-time PCR.Results:hUCWJMSCs grew faster than hPDLSCs.After osteogenic differentiation induction,hPDLSCs group showed higher ALP level,more mineralized nodule formation and higher Runx2 expression compared with hUCWJMSCs group (P ＜ 0.05);while the OPN expressed higher in hUCWJMSCs than in hPDLSCs (P ＜ 0.05).Conclusion:hUCWJMSCs and hPDLSCs have osteogenesis differentiation potential,hPDLSCs are more osteogenetic.