Effects of Rhizoma Alismatis decoction on lipid metabolism and PPARα and ACO expression in rats with hyperlipemia
10.3969/j.issn.1001-1978.2017.12.026
- VernacularTitle:泽泻汤对高脂血症大鼠脂代谢及PPARα 和ACO基因和蛋白表达的影响
- Author:
Xue HAN
1
;
qing Mu ZHANG
;
Bing CHANG
;
Xi WANG
;
Lei HAO
;
Yu LIU
;
Cheng SHI
;
hong Qiu GUO
;
xin Yi ZHANG
Author Information
1. 河北中医学院药学院临床中药学教研室
- Keywords:
Rhizoma Alismatis decotion;
hyperlipemia;
rats;
lipid meaboism;
PPARα;
ACO
- From:
Chinese Pharmacological Bulletin
2017;33(12):1761-1765
- CountryChina
- Language:Chinese
-
Abstract:
Aim To primarily discuss the mechanism of Rhizoma Alismatis decoction on prevention and treatment of hyperlipemia,through investigating hepat-ic expressions of peroxisome proliferator activated re-ceptor-α(PPARα)and acyl CoA oxidase (ACO)in rats with hyperlipemia. Methods Fifty male Sprague-Dawley rats(160 ~ 180 g)were randomly divided into five groups:control group,model group,high-dose of Rhizoma Alismatis decoction group (H-RAD),low-dose of Rhizoma Alismatis decoction group(L-RAD), and Xue-Zhi-Kang group(XZK). Rats in control group were fed with ordinary forage,and the others were with high-fat forage,which lasted for four weeks. At the same time,high and low-dose of Rhizoma Alismatis decoction,as well as Xuezhikang capsule,was admin-istered in respectively designed groups. Then,the TC, TG,HDL and LDL of serum were detected,the mor-phology of liver tissues was observed with HE,and the expressions of PPARα and ACO were detected with RT-PCR and Western blot after four weeks. Results Rhizoma Alismatis decoction could significantly reduce serum concentration of TC,TG and LDL(P < 0. 01), while increasing the concentration of HDL(P < 0. 01) and strengthening the expressions of PPARα and ACO (P < 0. 01). Conclusion Strengthening the expres-sions of PPARα and ACO can be viewed as mecha-nisms of Rhizoma Alismatis decoction in prevention and treatment of hyperlipemia.
