The effects of antimicrobial therapy on dendritic cells in the infected microenvironment of rabbit buccal VX-2 squamous cell carcinoma
10.3969/j.issn.1001-3733.2017.05.005
- VernacularTitle:抗菌治疗对兔颊VX-2鳞癌感染性微环境中树突状细胞的影响
- Author:
Zhihong CHEN
1
;
Jie YI
;
Guilin HUANG
;
Nini ZHANG
;
Li YAO
;
Ligang ZHANG
Author Information
1. 贵州省人民医院口腔科
- Keywords:
Antimicrobial therapy;
Inflammation;
VX-2 squamous cell carcinoma;
Dendritic cells
- From:
Journal of Practical Stomatology
2017;33(5):594-597
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of antimicrobial therapy on the maturation and function of dendritic cells(DCs) in the infected microenvironment of rabbit buccal VX-2 squamous cell carcinoma.Methods:The inflammatory models were obtained by mechanical trauma and high sugar diet on the basis of rabbit buccal VX-2 squamous cell carcinoma models which were established by particle implantation of the tumor tissue.The model rabbits were divided into 3 groups (n =6).In group A the rabbits with buccal VX-2 squamous cell carcinoma and local inflammation were given antibiotics by gavage and intramuscular injection for 3 consecutive days;the rabbits in group B with buccal VX-2 squamous cell carcinoma and local inflammation were given normal saline by gavage and intramuscular injection;the rabbits in group C with tumor and without inflammation were given normal saline by gavage and intramuscular injection.The tumor specimens were collected 3 days after treatment,and made into tissue homogenate,supernatant was collected after centrifugation.Normal rabbit peripheral blood mononuclear cells were separated and co-cultured with the supernatant obtained from the 3 groups respectively.Expressions of DCs surface markers HLA-DR,CD83 and CD86 were detected by flow cytometry.the function of DCs was tested by mixed lymphocyte reaction.Results:The positive rate of HLA-DR,CD83,CD86 and stimulate index were group C ＞ group A ＞ group B (P ＜ 0.05).Conclusion:Antimicrobial therapy can promote the maturation and function of DCs in the infected microenvironment of rabbit buccal VX-2 squamous cell carcinoma.
