The effects of β tricalcium phosphate/collagen scaffold loaded with human bone morphogenetic protein 2 plasmid on the osteogenesis ability of MC3T3-E1 cells
10.3969/j.issn.1001-3733.2017.05.006
- VernacularTitle:hBMP2修饰的β-TCP/胶原支架对MC3T3-E1细胞成骨能力的影响
- Author:
Gang ZHAO
1
;
Juan ZENG
;
Dechao LI
;
Yuansheng DING
;
Xujia ZHU
;
Tianxi SONG
Author Information
1. 佳木斯大学口腔医学院正畸科
- Keywords:
BMP2;
β-TCP/collagen;
Osteogenetic ability;
Bone defect
- From:
Journal of Practical Stomatology
2017;33(5):598-602
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of beta tricalcium phosphate(β-TCP)/collagen scaffold loaded with human bone morphogenetic protein 2 (hBMP2) plasmid on the osteogenesis ability of MC3T3-E1 cells.Methods:hBMP2 DNA plasmid-modified β-TCP/collagen scaffold and the naked plasmid(control) were constructed.MC3T3-E1 cells were respectively in vitro cultured onto the β-TCP/collagen scaffold with hBMP2(Z) and with control plasmid(Z0),on peace dish with the saffold and hBMP2(M) and with the control plasmid(M0).The surface morphology of the samples was observed by SEM.Osteogenesis of the cells was examined by alkaline phosphatase activity(ALP) test,real-time fluorescent quantitative PCR for the detection of Runx2,OCN,ALP and OPN mRNA expression.Data were statistically analyzed.Results:The composite sample surface of plasmid DNA containing hBMP2 modified β-TCP/collagen was porous;group Z and M showed highter ALP activity and higher mRNA expression of Runx2,OCN,ALP and OPN than group Z0 and M0;so did group Z than group M.Conclusion:Porous β-TCP/collagen scaffold loaded with BMP2 DNA is potential for osteoinduction.
