The effects of diameter-controlled titanium nanotubes on the proliferation, streching and collagen secretion of fibroblasts
10.3969/j.issn.1001-3733.2017.05.009
- VernacularTitle:不同管径钛纳米管对成纤维细胞增殖、伸展和胶原分泌功能的影响
- Author:
Hongcai LI
1
;
Zhuang MA
Author Information
1. 株洲市中心医院口腔科
- Keywords:
Nanotubes;
Fibroblasts;
Cell proliferation;
Cell spreading;
Collagen fiber
- From:
Journal of Practical Stomatology
2017;33(5):612-616
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of Ti-TiO2 nanotubes with different diameters on the proliferation,stretching and collagen secretion of fibroblasts.Methods:TiO2 nanotubes formed at 1,5,10 and 20 V potentials served as the experimental samples and polished pure titanium served as the control.Fibroblasts was cultivated on the surface of the various samples.MTT assay was used to examine the cell proliferation.The surface morphology of the cells was observed with SEM.Collagen secrection was tested by sirius red/bitter acid staining.Results:The nanotubes prepared by 1,5,10 and 20 V were with the diameter of 15,30,50 and 100 nm respectively.At day 1,3 and 5,the cell proliferation on polished pure titanium surface was more than that on the nanotubes surfaces at the same time;at day 5,cell proliferation on 100 nm nanotubes was significantly more than that on the other nanotube surfaces (P ＜ 0.01).At day 3,fibroblasts at polished pure titanium surface stretched as typical long spindle form,while with obvious pseudopodium at nanotube surfaces.The collagen secretion of fibroblasts was highest at 100 nm nanotubes (P ＜ 0.01).Conclusion:100 nm nanotubes may have minimal negative effect on fibroblast proliferation and the greatest positive effect on the collagen secretion.
