A primary study of the differential proteomic expression in saliva of health people and the patients with oral squamous cell carcinoma

VernacularTitle:口腔鳞癌患者与健康人唾液差异蛋白组学的初步研究
Author: Kunjun SUN ¹ ; Hong MA ; Yingqian KANG ; Xianyu ZOU
Author Information

1. 贵州医科大学口腔医学院口腔颌面外科学教研室
Keywords: Saliva; Oral Squamous cell carcinoma (OSCC); Proteomics; Two-dimensional gel electrophoresis (2-DE); Tumor marker
From: Journal of Practical Stomatology 2017;33(5):653-656
CountryChina
Language:Chinese
Abstract: Objective:To study the differentially expressed proteins in saliva of health people and the patients with oral squamous cell carcinoma(OSCC).Methods:Saliva of 17 cases with OSCC and paired health subjects was collected,the proteins in the saliva were examined by two-dimensional gel electrophoresis (2-DE) separation,the proteins were examined by 2-DE separation,the saliva proteome dimensional electrophoresis profiles were obtained by MALDI-TOF/MS mass spectrometric identification,the information of the differentially expressed protein in OSCC group was studied by NCBI database bioinformatics analysis.Results:10 proteins differentially expressed between the 2 groups were observed by mass spectrometry.Bioinformatics analysis showed that S100A8,S100A8/S100A9 and Epidermal cytokeratin 2(EK2) were highly expressed in the saliva of OSCC cases.Conclusion:S100A8,S100A8/S100A9 and EK2 may be related to the development of OSCC.