Bigelovii E,a triterpenoid compound of Salicornia bigelovii,induced apoptosis of breast cancer MCF-7 cells via inhibiting mTOR / P70S6K / 4-EBP signaling
10.3969/j.issn.1001-1978.2017.12.007
- VernacularTitle:北美海篷子三萜皂苷bigelovii E抑制mTOR信号通路诱导乳腺癌MCF-7细胞凋亡
- Author:
zhen Zhen HUANG
1
;
yu Dan ZHANG
;
Qi SHI
;
qin Fu GUAN
;
zhi Qi WANG
;
Ming WANG
;
Xu FENG
;
Yu SHAN
Author Information
1. 江苏省中国科学院植物研究所
- Keywords:
Salicornia bigelovii;
bigelovii E;
breast cancer;
apoptosis;
mTOR;
anti-tumor
- From:
Chinese Pharmacological Bulletin
2017;33(12):1655-1660
- CountryChina
- Language:Chinese
-
Abstract:
Aim To demonstrate that bigelovii E,one of the triterpenoid compounds isolated from Salicornia bigelovii Torr. ,induced apoptosis and inhibited prolif-eration of human breast cancer cells MCF-7. Methods MTT and Clonogenic were used to detect the anti-proliferative effect of bigelovii E on human tumor cells. Hoechst 33258 staining was used to observe the chan-ges of cell morphology in the treatment with bigelovii E. The effect of bigelovii E on cell apoptosis,cell mi-tochondrial membrane potential and the ROS level was analyzed by flow cytometry. The expression of mito-chondrial apoptotic pathway and its regulated proteins were analyzed by Western blot. Results Bigelovii E was most sensitive to MCF-7;bigelovii E had lower toxicity to normal cells HLF1;bigelovii E inhibited the colony formation of breast cancer MCF-7;the apoptosis pattern induced by bigelovii E was detected by Hoechst 33258;Western blot showed that bigelovii E could down-regulate the phosphorylation of mTOR,p70S6K and 4-EBP,up-regulate the expression of Bcl-xl and Mcl-1 proteins,promote the mitochondrial apoptosis pathway,reduce the mitochondrial membrane poten-tial,and induce ROS,leading to mitochondrial func-tion damage,and ultimately inducing apoptosis. Con-clusion Bigelovii E regulates mitochondrial apoptosis pathway through mTOR pathway,induces apoptosis and has a good anti-tumor effect.