Role of endoplasmic reticulum stress signaling pathway in high fat-induced cell apoptosis in vascular endothelial cells
10.3969/j.issn.1001-1978.2017.12.009
- VernacularTitle:内质网应激信号通路在棕榈酸诱导的血管内皮细胞凋亡中的作用
- Author:
hu Wen XU
1
;
zi Chun JIN
;
long Xiao WANG
;
Chen CHEN
;
Lan CUI
Author Information
1. 延边大学附属医院心血管内科
- Keywords:
endoplasmic reticulum stress;
palmitic acid;
vascular endothelial cell;
4-phenylbutyric acid;
human umbilical vein endothelial cells;
cell apoptosis
- From:
Chinese Pharmacological Bulletin
2017;33(12):1668-1672
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the role of endoplasmic reticulum stress(ERS)signaling pathway in high fat-induced cell apoptosis in human umbilical vein endo-thelial cells(HUVECs). Methods HUVECs were ex-posed to different concentrations of palmitic acid(0. 1, 0. 2,0. 4,0. 8 mmol·L - 1 )for 24 h and different time points of 0. 4 mmol·L - 1 palmitic acid(0,12, 24,48 h). Cell viability was measured by cell count-ing kit(CCK-8),and the protein expressions of ERS signaling pathway protein such as GRP78,CHOP, PERK,IRE1,ATF6 were determined by Western blot. The level of intracellular apoptosis was detected by immunofluorescence. Results HUVECs exposed to palmitic acid at 0. 4 mmol·L - 1 for 24 h showed a de-crease in their viability and an increase in the expres-sion of ERS signaling pathway proteins (GRP78, CHOP,PERK,IRE1,ATF6)(P < 0. 05);cell ap-optotic levels significantly increased(P < 0. 05). The intracellular apoptosis levels in the vascular endothelial cells of ERS signaling pathway inhibitor 4-phenylbutyr-ic acid (4-PBA,10 mmol · L - 1 )were significantly lower than those of the PA group(P < 0. 05). Conclu-sion Activated ERS signaling pathway might play an important role in the treatment of high fat-induced cell apoptosis in vascular endothelial cells.
