Effects of aralosdie C on excitation-contraction coupling of ischemia / reperfusion cardiomyocytes and role of CaMK II regulation
10.3969/j.issn.1001-1978.2017.12.011
- VernacularTitle:楤木皂苷C对缺血/再灌注原代大鼠心肌细胞兴奋收缩偶联及CaMKⅡ的调控作用
- Author:
bin Yu JI
1
;
song Xue LIU
;
di Miao ZHANG
;
Min WANG
;
yi Jing ZHANG
;
ying Rui WANG
;
bo Gui SUN
;
bo Xiao SUN
Author Information
1. 哈尔滨商业大学生命科学与环境科学研究中心
- Keywords:
aralosdie C (SMC );
cardiomyocyte;
CaMK II;
ischemia / reperfusion;
cell contraction and ion concentration synchronization determination sys-tem;
cell contraction and calcium transient
- From:
Chinese Pharmacological Bulletin
2017;33(12):1678-1684
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of aralosdie C (SMC)isolated from aralosides on contractile func-tion and calcium transients in ischemia / reperfusion(I/R)-induced rat myocardial injury and the role of CaMK II. Methods The cardiomyocytes were divided into control group,I/ R group,I/ R + SMC group,KN-93+ I/ R + SMC group. The effect of SMC on the con-tractile function and calcium transient of I/ R cells was measured by cell shrinkage and ion concentration sim-ultaneous detection system. Results SMC(8 μmol· L - 1 )improved the contractile function of I/ R cardio-myocytes and the calcium transients,and SMC in-creased the rate of calcium transient and [Ca2 +]i up /down regulation significantly. While CaMK II was blocked by KN-93,the effect of SMC on contractile function and calcium transients was weakened. Con-clusions SMC can significantly improve the systolic function and calcium transients of cardiomyocytes in I/R rats,and the protective effect of SMC on cardiomyo-cytes may be related to CaMK II.
