Effect of miR-185 targeting Six1 on epithelial-mesenchymal transition in lung cancer and its mechanism
10.13315/j.cnki.cjcep.2017.08.011
- VernacularTitle:miR-185靶向Six1对肺癌上皮-间质转化过程的影响及其机制
- Author:
fei Yan CAO
1
;
Rui REN
;
Ye YANG
;
hui Xiang LUO
;
li Shui WANG
Author Information
1. 陕西省人民医院胸外科
- Keywords:
lung neoplasms;
migration;
invasion;
miR-185;
Six1
- From:
Chinese Journal of Clinical and Experimental Pathology
2017;33(8):879-883
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To study the molecular mechanism of miR-185 affecting the migration and invasion of human lung cancer cell.Methods MiR-185 overexpression was obtained by transfection of miR-185 mimic in lung squamous cell carcinoma cell line H520 and A549,transwell assay and cell scratch assay were used to detection of cell migration and invasion.The luciferase reporter assay confirmed that miR-185 targets the Six1 gene.qRT-PCR and Western blot were used to detect the impact of miR-185 cells Six1 gene expression.Western blot was used to detect the effect of miR-185 overexpression on the epithelialmesenchymal transition of lung cancer cells.Results miR-185overexpression reduced migration and invasion of lung cancer cells (P ＜ 0.05),increased epithelial cell marker E-cadherin expression (P ＜ 0.01),and decreased the expression of mesenchymal cell markers vimentin of (P ＜ 0.01).After overexpression of miR-185 in H520 cells,the expression level of Six1gene was reduced (P＜0.01).MiR-185 regulated the migration and invasion of lung cancer cells by targeting the Six1 gene.Conclusion MiR-185 targets the Six1 gene to regulate the EMT pathway of human lung cancer cells.
