Optimization of purification technology for polyphenols from vitis amurensis seed extract with macroporous resins
10.13481/j.1671-587x.20170639
- VernacularTitle:大孔树脂对山葡萄籽多酚提取物的纯化工艺优选
- Author:
Hong JI
1
;
Rong JIA
;
Xing GUO
Author Information
1. 吉林大学中日联谊医院药学部
- Keywords:
vitis amurensis seeds;
polyphenols;
macroporous resin;
purification
- From:
Journal of Jilin University(Medicine Edition)
2017;43(6):1272-1277
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the purification technology parameters for polyphenols from vitis amurensis seed extract with AB-8 macroporous resin and to establish the optimum purification process conditions,and to provide technical reference for further development and utilization of vitis amurensis seeds.Methods:The vitis amurensis seeds were regarded as raw material,the concentration of vitis amurensis seed polyphenols was detected, and the adsorption rate and desorption rate of polyphenols were calculated.The AB-8 macroporous resin was used to purify the polyphenols from vitis amurensis seed extract, and the purification technology parameters of vitis amurensis seed polyphenols were optimized by absorption-desorption experiment.The concentrations of polyphenol from vitis amurensis seed extract were compared between before and after purification, and the effect of the purification process was verified. Results: The AB-8 macroporous resin had good absorption and desorption properties to crude the extract of vitis amurensis seed polyphenols,and the adsorption rate and desorption rate were 90.48% and 71.42%.The optimum adsorption conditions and desorption conditions were as follows:the sample concentration was 10 g· L-1 ,the sample flow rate was 3 mL· min-1 ,the eluting concentration was 60%,the elution velocity was 2 mL·min-1 ,and the eluting volume was 2-fold column volume.Under this condition,the concentration of polyphenols from vitis amurensis seed extract after purification was increased from 35.02% to 88.8%.Conclusion:AB-8 macroporous resin has better purification effect,and it is worthy of popularization and application.
