Establishment and Analysis for Differential Gene Expression Profile of Left Atrium in Permanent Atrial Fibrillation Patients
10.3969/j.issn.1000-3614.2017.11.010
- VernacularTitle:永久性心房颤动患者左心房差异基因表达谱的建立与分析
- Author:
Jie GAO
1
;
Jian ZHOU
;
xiong Pi SU
;
Yan LIU
;
Song GU
;
tao Xi ZHANG
;
guang Xiang AN
Author Information
1. 首都医科大学附属北京朝阳医院 心外科
- Keywords:
Atrial fibrillation;
Gene expression profile
- From:
Chinese Circulation Journal
2017;32(11):1085-1090
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the relevant gene, signaling pathway for permanent atrial fibrillation (pAF) occurrence in order to provide the molecular basis of the pathogenesis of pAF. Methods: Our research included in 2 groups: pAF group, n=7 patients and Control group, n=4 healthy subjects with sinus rhythm. Agilent 4x44K microarray was used to analyze the mRNA in left atrium for differential gene expression profile. Based on Gene Ontology, KEGG and Biocarta databases, differentially expressed genes were studied for their relevant function and signaling pathway. Furthermore, the genes with significant differences were verified by quantitative real time PCR (qRT-PCR) in pathological specimen from 5 pAF patients and 5 normal heart donors. Results: The expression profile identified 987 abnormally expressed genes, 567 of them were down-regulated and 420 were up-regulated. 9 genes with significant differences were verified by qRT-PCR in pathological specimen and the changes were similar to microarray; those genes were closely related to pAF by involving left atrium fibrosis, electrical remodeling, inflammation, cellular stress response, metabolism and transcription regulation. GO and Pathway analysis indicated that down-regulated genes were mainly involved in metabolic processes; up-regulated genes had the effects on cellular stress response, immune response and platelet activation. Conclusion: Microarray technology identified some important genes related to pAF occurrence; such genes involved in left atrial structural and functional remodeling via affecting cellular metabolism, inflammation, immune response and thrombogenesis in relevant patients.
