Protective effects of SFE-CO2 of Notoginseng against glutamate-induced apoptosis in PC12 cells
10.3969/j.issn.1001-1978.2017.12.012
- VernacularTitle:三七超临界CO2萃取物对谷氨酸损伤PC12细胞的保护作用
- Author:
chun Xian DUAN
1
;
An ZHOU
;
yin Dai PENG
;
yun Jin BAO
;
zhu Lun XIA
Author Information
1. 安徽中医药大学药学院
- Keywords:
Notoginseng SFE;
glutamate;
PC12 cells;
Ca2+;
PICK1;
GluR2
- From:
Chinese Pharmacological Bulletin
2017;33(12):1685-1690
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the protective effects of supercritical CO2 fluid extract(SFE)of Notoginseng a-gainst glutamate-induced PC12 cells damage and the underlying mechanism. Methods PC12 cells were dealt with glutamate to establish cell models. MTT as-say,LDH method,Hoschst 33342 staining,Fluo-3 /AM fluorescence staining and Western blot were used to observe the changes of cell viability,intracellular Ca2 + concentration and the expression of protein that interacted with C kinase l(PICK1)and glutamate re-ceptors 2 (GluR2),respectively. Results Glutamate was cytotoxic to PC12 cells with an inhibitory concen-tration 50(IC 50 )of 25 mmol·L - 1 . Pretreatment with SFE(25,50,100 mg·L-1)and FSC231(100 μmol ·L-1 )and SFE(100 mg·L-1 )+FSC231(100μmol ·L-1 )remarkablely improved cell viability,reduced LDH leakage,decreased apoptosis rate,debased intra-cellular calcium concentration,decreased the expres-sion of PICK1 ,and increased the expression of GluR2 . Conclusions SFE of Notoginseng shows protective effects against glutamate-induced PC12 cell damage, and its mechanism may be related to the inhibition of PICK1 and the increase of GluR2 protein expression.
