Relevant research of PD-1, PD-L1 protein expression and K-RAS gene mutations in lung adenocarcinoma
10.13315/j.cnki.cjcep.2017.07.011
- VernacularTitle:肺腺癌中PD-1、PD-L1蛋白表达与K-RAS基因突变状态的相关性分析
- Author:
Shan WANG
1
;
ru Li DONG
;
qiang Hui REN
;
dong Ai LIU
;
jie Yan XIONG
;
dong Xu SONG
Author Information
1. 河北省唐山市华北理工大学附属医院病理科 063000
- Keywords:
lung neoplasm;
adenocarcinoma;
PD-1;
PD-L1;
K-RAS gene mutations
- From:
Chinese Journal of Clinical and Experimental Pathology
2017;33(7):754-758
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the association of the PD-1 and PD-L1 protein expression with K-RAS gene mutations in lung adenocarcinoma.Methods The protein expression of PD-1 and PD-L1 was detected by immunohistochemical EnVision two-step staining,the K-RAS mutation was examined by realtime fluorescent quantitative PCR.Results The positive rate of PD-1 and PD-L1 was higher in lung adenocarcinoma than benign lung disease (P ＜ 0.01).There was no relationship between PD-1 and PD-L1 protein expression with the gender,age,smoking condition,differentiation,lymph node metastasis and TNM stages (P ＞ 0.05).The K-RAS gene mutations were detectable in 8 patients (22.2％) among 36 lung adenocarcinoma,there was no association between K-RAS gene mutation with the gender,age,smoking condition,differentiation,lymph node metastasis and TNM stages (P ＞ 0.05).The correction analysis showed that there was no relationship between PD-1 and PD-L1 protein expression with K-RAS mutation (P ＞ 0.05).Conclusion The positive rate of PD-1 and PD-L1 is higher in lung adenocarcinoma than benign lung disease,but there is no relationship among PD-1 and PD-L1 protein expression with its clinal pathological characteristics and K-RAS mutation in lung adenocarcinoma.
