Expression of Uba-2 in hepatic carcinoma and its effect on the proliferation, invasion and metastasis of hepatoma cells
10.13315/j.cnki.cjcep.2017.08.003
- VernacularTitle:Uba-2在肝癌组织中的表达及其对肝癌细胞增殖和侵袭转移能力的影响
- Author:
Zheng YUAN
1
;
Zhen LI
;
zhen Yan SUN
Author Information
1. 河南省南阳市中心医院病理科
- Keywords:
hepatic neoplasms;
cell proliferation;
invasion and metastasis;
Uba-2
- From:
Chinese Journal of Clinical and Experimental Pathology
2017;33(8):837-840,846
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the effect of differential expression,over expression and low expression of Uba-2 in hepatic carcinoma on the proliferation and invasion of hepatoma cells.Methods The expression of Uba-2 mRNA in clinical specimens was detected by qRT-PCR.Mter transfection of Uba2 over expression vector and siRNA expression vector into hepatoma cell HepG2,the effect of Uba-2 on proliferation of hepatoma cells was detected by MTS assay while the effect on the invasion and metastasis of hepatoma cells was detected by cell migration/invasion assay.The expression levels of TGF-β1-3,VEGF and Snail proteins in hepatoma cell HepG2 were detected by Western blot.Results The expression of Uba-2 was high in hepatic carcinoma.Compared with that in adjacent liver tissues,there were significant differences (P ＜ 0.01).qRT-PCR detected over expression or low expression of Uba-2 in HepG2 cells.Cell proliferation data showed that the expression level of Uba-2 was related to the proliferation ability of hepatoma cell HepG2,and siRNA interferring the expression of Uba-2 in silent HepG2 cells could inhibit its growth.The data of cell migration/invasion showed that over expression of Uba-2 could promote the invasion and metastasis of hepatoma cell HepG2.Compared with cells in the control group,there were statistically significant differences.The results of Western blot indicated that the abnormal expression of Uba-2 affected the expression levels of TGF-β2,VEGF and Snail proteins.Conclusion The high expression of Uba-2 gene in hepatic carcinoma may promote the proliferation,invasion and metastasis of hepatoma cells.
