Research on the quality standard of Baoyun pill

VernacularTitle:保孕丸质量标准研究
Author: Yi LU ¹ ; Jing DUAN ; Yawei WANG ; Yanping ZHOU ; Jing MA ; Lianghong SI
Author Information

1. 解放军第454医院药剂科
Keywords: Chromatography,thin layer; Chromatography,high pressure liquid; Quality control; Hesperidine
From: International Journal of Traditional Chinese Medicine 2017;39(11):1016-1019
CountryChina
Language:Chinese
Abstract: Objective To improve the quality standard of Baoyun pill. Methods The natures of Psoralea corylifolia, Radix Dipsaci and Loranthus parasiticus were detected by thin layer chromatography (TLC); the content of Hesperidine were measured by high performance liquid chromatography (HPLC). The operating conditions by HPLC were InertSustain C18 (4.6 mm×250 mm, 5 μm ), the mixture of methanol-acetic acid-water (35:4:61) as mobile phase, 1.0 ml/min as flow speed and wavelength 283 nm. Results The results showed that the TLC spots of Psoralea corylifolia, Radix Dipsaci and Loranthus parasiticus were fairly clear, and the according blank test showed no interference. Hesperidine showed a good linear relationship within the range of 0.820-4.920 μg(r=1.000 0)with an average recovery of 99.85%(RSD=0.21%).Conclusions The TLC and HPLC are handy,safe and accurate for Baoyun pills to determine the quality.