Effects of Ganlu Xiaodu Pills and Its Residues on PSGL-1 and Proinflammatory Cytokines in CoxA16 Mouse Model
10.3969/j.issn.1005-5304.2017.12.011
- VernacularTitle:甘露消毒丹及其拆方对柯萨奇病毒A16小鼠模型P-选凝素糖蛋白配体1和促炎因子的影响
- Author:
ju Jun ZOU
1
;
min Jia WU
;
shun You HE
;
rong Can WU
;
rong Guo ZHAO
Author Information
1. 湖南中医药大学中医学院
- Keywords:
Ganlu Xiaodu Pills;
residual;
PSGL-1;
proinflammatory cytokines;
mice
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2017;24(12):42-46
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Ganlu Xiaodu Pills and its residues on PSGL-1 and proinflammatory cytokines in Coxsackie virus A16 (CoxA16) mouse model; To discuss its antiviral mechanism of action. Methods Totally 150 ICR mice at age of 7 days were randomly divided into normal group, model group, all-side group, aromatic residual group, clearing residual group and removing residual group, with 25 mice in each group. Except for normal group, other groups were injected intraperitoneally with 20 μL of 107TCID50 CoxA16 standard stock solution to establish models. Except for normal group and model group, other groups were given relevant medicine for intervention. The expressions of PSGL-1, TNF-α, IL-1β and IL-4 and histopathological observation were detected after 10 days of medication. Results Except for the normal group, the existence of a large number of CoxA16 in other groups of mouse muscle tissues proved successful modeling. HE staining showed that Ganlu Xiaodu Pills and residual could reduce damage to the muscle by CoxA16 virus. Compared with the normal group, the expression of PSGL-1 protein in the model group increased (P<0.01); compared with the model group, all-side group, aromatic residue group, clearing residual group, removing residual group inhibited the expression of PSGL-1 protein, reduced the inflammatory factors of TNF-α, IL-1β, and IL-4 (P<0.01). Conclusion Ganlu Xiaodu Pills and its residues have anti-inflammatory effects, and the all-side group shows the best efficacy.
