Study on UPLC Fingerprints of Lianhua Qingwen Capsules
10.3969/j.issn.1005-5304.2017.12.019
- VernacularTitle:连花清瘟胶囊超高效液相指纹图谱研究
- Author:
peng Yu CHEN
1
;
Qian ZHAO
;
jin Gui WANG
;
ying Shui ZHANG
;
Dan BI
Author Information
1. 河北省中西医结合医药研究院
- Keywords:
Lianhua Qingwen Capsules;
UPLC;
fingerprints
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2017;24(12):77-80
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study and establish the UPLC fingerprints of Lianhua Qingwen Capsules. Methods The samples were separated with a Waters ACQUITY UPLC HSS T3 column (100 mm × 2.1 mm, 1.8 μm) by linear gradient elution. The wavelength for detection was set at 239 nm; mobile phase was set at a flow rate of 0.3 mL/min;the column temperature was set at 30 ℃. Results UPLC fingerprints of Lianhua Qingwen Capsules were established with 32 common peaks. 9 of 32 common peaks were identified, including neochlorogenic acid (peak No.4, source from Lonicerae Japonicae Flos and Houttuyniae Herba), chlorogenic acid (peak No.6, source from Forsythiae Fructus, Lonicerae Japonicae Flos and Houttuyniae Herba), cryptochlorogenic acid (peak No.8, source from Lonicerae Japonicae Flos and Houttuyniae Herba), isoforsythiaside A (peak No.15, source from Forsythiae Fructus), forsythoside A (peak No.20, source from Forsythiae Fructus), quercitrin (peak No.23, source from Houttuyniae Herba), isochlorogenic acid C (peak No.24, source from Lonicerae Japonicae Flos), phillyrin (peak No.26, source from Forsythiae Fructus), glycyrrhizic acid (peak No.31, source from Glycyrrhizae Radix et Rhizoma). The similarities in 10 batches of Lianhua Qingwen Capsules samples were all above 0.96. Conclusion The method is with good precision, repeatability and stability, which can be used as a new means for the quality control of Lianhua Qingwen Capsules.
