Comparative Study on Transformation of Tecomin by Using Liver Microsomes and Liver S9 QIAO Shan-shan,

VernacularTitle:肝微粒体和肝S9转化藜芦酸葡萄糖酯比较研究
Author: shan Shan QIAO ¹ ; qi Shi ZHENG ; yue Ming FANG ; zhi Duo SHI ; li De LI ; yu Teng WANG ; feng Ru WANG
Author Information

1. 北京中医药大学生命科学学院
Keywords: tecomin; veratric acid; liver microsomes; liver S9 fraction
From: Chinese Journal of Information on Traditional Chinese Medicine 2017;24(12):60-63
CountryChina
Language:Chinese
Abstract: Objective To compare the difference of transformation profile and transformation rate of tecomin by using two in vitro liver metabolism models. Methods Liver microsomes and liver S9 fraction models were employed to transform tecomin. HPLC was used to determine the contents of tecomin and its metabolites at the detecting wavelength of 254 nm. The gradient elution (0–6 min, 5％–40％ A; 6–9 min, 40％–50％ A; 9–11 min, 50％–5％ A) was carried out by using mobile phase of acetonitrile (A) - 1％ acetic acid (B) at a flow rate of 1 mL/min. Results Both models could transform tecomin into veratric acid; however, the metabolites obtained with liver S9 were more than those obtained with liver microsomes, and the transformation rate of the former was higher than that of the latter. Conclusion The liver S9 fraction can more efficiently transform esters than liver microsomes.